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    发布日期:2018-07-11

详细介绍

pJF3H cDNA展示系统

货号 PDS27-9401-08
【系统说明】

pJF3H cDNA phage display system is the best platform for preparing cDNA library. pJF3H utilizes the backbone of pComb3H and a specially designed cassette for cDNA library display. The cDNA library is expressed as a C-Terminal fusion on Fos. The pIII gene product is expressed as a C-Terminal fusion to Jun. The ompA and pelB leader sequences direct the products to the periplasm where they assembly via Jun/Fos interaction.

【系统内容】

A.表达宿主菌E.coli  XL1-Blue15%甘油保存),800ul/支;

B.噬菌粒载体pComb3 lambdaDNA溶于TE Buffer,浓度50ng/ul),30ul/支;

C.辅助噬菌体VCSM13(滴度为1.2×1013pfu/ml),800ul/支;

D. 可溶性表达宿主菌E.coli HB215115%甘油保存),800ul/支;
E.《运用pJF3H 展示系统构建cDNA文库实用操作手册》

【保存温度】

噬菌粒载体、宿主菌保存于-20℃。

辅助噬菌体请存放在4℃即可,不可冻存

【载体信息】

BACKBONE

Vector backbonepComb3H

Backbone size w/o insert (bp)3300

Total vector size (bp)3900

Vector typeBacterial Expression ; phage display

GROWTH IN BACTERIA

Bacterial Resistance(s)Ampicillin

Growth Temperature37°C

Growth Strain(s)XL1-BlueT+

Copy numberHigh Copy

GENE/INSERT

Gene/Insert namecDNA cloning region

SpeciesSynthetic

Insert Size (bp)200

PromoterlacZ

Tag / Fusion ProteinFos (N terminal on backbone)

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ cloning siteBssHI (not destroyed)

3′ cloning siteNotI (not destroyed)

5′ sequencing primerAAG ACA GCT ATC GCG ATT GCA G

3′ sequencing primerACC TAT TGC CTA CGG CAG CCG


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