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详细介绍
pJF3H cDNA展示系统
货号 PDS27-9401-08
【系统说明】
pJF3H cDNA phage display system is the best platform for preparing cDNA library. pJF3H utilizes the backbone of pComb3H and a specially designed cassette for cDNA library display. The cDNA library is expressed as a C-Terminal fusion on Fos. The pIII gene product is expressed as a C-Terminal fusion to Jun. The ompA and pelB leader sequences direct the products to the periplasm where they assembly via Jun/Fos interaction.
【系统内容】
A.表达宿主菌E.coli XL1-Blue(15%甘油保存),800ul/支;
B.噬菌粒载体pComb3 lambda(DNA溶于TE Buffer,浓度50ng/ul),30ul/支;
C.辅助噬菌体VCSM13(滴度为1.2×1013pfu/ml),800ul/支;
D. 可溶性表达宿主菌E.coli HB2151(15%甘油保存),800ul/支;
E.《运用pJF3H 展示系统构建cDNA文库实用操作手册》
【保存温度】
噬菌粒载体、宿主菌保存于-20℃。
辅助噬菌体请存放在4℃即可,不可冻存。
【载体信息】
BACKBONE
•Vector backbone:pComb3H
•Backbone size w/o insert (bp):3300
•Total vector size (bp):3900
•Vector type:Bacterial Expression ; phage display
GROWTH IN BACTERIA
•Bacterial Resistance(s):Ampicillin
•Growth Temperature:37°C
•Growth Strain(s):XL1-Blue,T+
•Copy number:High Copy
GENE/INSERT
•Gene/Insert name:cDNA cloning region
•Species:Synthetic
•Insert Size (bp):200
•Promoter:lacZ
•Tag / Fusion Protein:Fos (N terminal on backbone)
CLONING INFORMATION
•Cloning method:Restriction Enzyme
•5′ cloning site:BssHI (not destroyed)
•3′ cloning site:NotI (not destroyed)
•5′ sequencing primer:AAG ACA GCT ATC GCG ATT GCA G
•3′ sequencing primer:ACC TAT TGC CTA CGG CAG CCG