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详细介绍
pComb3 HTT展示系统
货号 PDS27-9401-07
【系统说明】
pComb3H is the second generation of pComb vector. Improvements over pComb3 include increased stability and introduction of a new cloning region utilizing asymmetrical SfiI restriction sites for directional cloning of full Fab, scFv, peptide and other proteins for phage display. The “TT” refers to the human Fab to tetanus toxin which can be used as a phage display and protein expression control. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
【系统内容】
A．表达宿主菌E.coli XL1-Blue（15％甘油保存），800ul/支；
B．噬菌粒载体pComb3 HTT（DNA溶于TE Buffer，浓度50ng/ul），30ul/支；
C．辅助噬菌体VCSM13（滴度为1.2×1013pfu/ml），800ul/支；
D. 可溶性表达宿主菌E.coli HB2151（15%甘油保存），800ul/支；
E.《运用pcomb3HTT展示系统建库适用操作手册》
【保存温度】
噬菌粒载体、宿主菌保存于-20℃。
辅助噬菌体请存放在4℃即可，不可冻存。
