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    发布日期:2018-07-12

详细介绍

Phagemid pComb3 Lambda

Cat No. PM 13006

DEPOSITOR COMMENTS

pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XLambda can be used as a template to amplify the lambda light chain constant region for construction of chimeric antibody libraries.

GROWTH IN BACTERIA

Bacterial Resistance(s)Ampicillin

Growth Temperature37°C

Growth Strain(s)XL1 Blue

Copy numberHigh Copy

GENE/INSERT

Gene/Insert namehuman antibody lambda light chain

SpeciesH. sapiens (human)

Insert Size (bp)320

Entrez GeneIGLL5 (a.k.a. IGL, IGLV, VL-MAR)

PromoterlacZ

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ cloning siteSfiI (not destroyed)

3′ cloning siteSfiI (not destroyed)

5′ sequencing primerAAG ACA GCT ATC GCG ATT GCA G

3′ sequencing primerGCC CCC TTA TTA GCG TTT GCC ATC


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