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    发布日期:2018-07-11

详细介绍

Phagemid pcomb3 HTT

Cat No. PM13007

DEPOSITOR COMMENTS

pComb3H is the second generation of pComb vector. Improvements over pComb3 include increased stability and introduction of a new cloning region utilizing asymmetrical SfiI restriction sites for directional cloning of full Fab, scFv, peptide and other proteins for phage display. The “TT” refers to the human Fab to tetanus toxin which can be used as a phage display and protein expression control. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.

BACKBONE

Vector backbone: pComb3H

Backbone size w/o insert (bp): 3300

Total vector size (bp): 4700

Vector type: Bacterial Expression ; phage display

GROWTH IN BACTERIA

Bacterial Resistance(s): Ampicillin

Growth Temperature: 37°C

Growth Strain(s): XL1-Blue

Copy number: High Copy

GENE/INSERT

Gene/Insert name: TT Fab

Alt name: human Fab to tetanus toxin

Species: H. sapiens (human)

Insert Size (bp):1400

Promoter: lacZ

Tag / Fusion Protein: geneIII (C terminal on backbone)

CLONING INFORMATION

Cloning method: Restriction Enzyme

5′ cloning site: SfiI (not destroyed)

3′ cloning site: SfiI (not destroyed)

5′ sequencing primer: 5'-AAG ACA GCT ATC GCG ATT GCA G-3'

3′ sequencing primer: 5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'


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